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Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
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产品介绍
产品介绍
产品信息
抗原名称
C-C motif chemokine 4
商品描述
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIP1b in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MIP1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MIP1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
应用
反应种属
Rat
检测范围
78.125-5000pg/mL
检测类型
Sandwich
样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
敏感性
30pg/mL
背景
别名
CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
116637
UniProt ID
P50230
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