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Rat Ubiquitin (Ub) ELISA Kit
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产品介绍
产品介绍
产品信息
抗原名称
ubiquitin
商品描述
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Ub in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ub has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ub and unlabeled Ub (Standards or samples) with the pre-coated antibody specific to Ub. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ub in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ub in the sample.
应用
反应种属
Rat
检测范围
6.25-400ng/mL
检测类型
Competitive inhibition
样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
敏感性
3.6ng/mL
背景
别名
Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
7316
UniProt ID
P0CG48
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