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Rat Vitronectin (VTN) ELISA Kit
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产品介绍
产品信息
抗原名称
vitronectin
商品描述
Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VTN in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VTN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
应用
反应种属
Rat
检测范围
78.12-5000ng/mL
检测类型
Sandwich
样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
敏感性
31ng/mL
背景
别名
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
29169
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