首页
rhLatent Activin A, (25 ug)
rhLatent Activin A, (25 ug)

rhLatent Activin A, (25 ug)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    产品介绍
    产品信息

    Scientific Data

    Bioactivity Recombinant Human Latent Activin A Protein BioactivityView Larger

    Recombinant Human Latent Activin A (Catalog # 9129-LA) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 is 0.600-3.60 ng/mL.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9129-LA

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 100 μg/mL in PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human Latent Activin A Protein, CF Summary

    Product Specifications

    Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.600‑3.60 ng/mL.
    Source
    Chinese Hamster Ovary cell line, CHO-derived human Activin A protein
    Ser21-Ser426
    Accession #
    P08476
    N-terminal Sequence
    Analysis
    Ser21 (pro) & Gly311 (mature)
    Structure / Form
    Noncovalently-linked complex between disulfide-linked homodimer of the mature domain and noncovalently-linked homodimer of the prodomain
    Predicted Molecular Mass
    32 kDa (pro) & 13 kDa (mature)
    SDS-PAGE
    13-16 kDa (mature) and 39 - 46 kDa (pro), reducing conditions
    背景
    别名
    Activin A,activin AB alpha polypeptide,Activin beta-A chain,erythroid differentiation factor,Erythroid differentiation protein,follicle-stimulating hormone-releasing protein,FSH-releasing protein,inhibin beta A chain,inhibin beta A subunit,Inhibin, beta-1
    背景

    Background: Activin A

    Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. 


    Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell‑associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16). 

    Activin A is involved in the differentiation of various cell and tissue types. The induction of definitive endoderm by Activin A is required in differentiation protocols of induced pluripotent stem cells (iPSCs) (17, 18). In vitro models of human gametogenesis use prolonged Activin A supplementation to human embryonic stem cells for differentiation into human primordial germ cell-like cells (19). Activin A can also be used to maintain cells in vitro, as is the case for iPSC-derived nephron cells that can then be used in disease modeling, drug screening and in regenerative medicine (20). 

    Activin A is an important factor for tumor cells to evade the immune system as Activin A can act on surrounding immune cells to decrease their antitumor activity (21). Activin A also promotes migration and growth of tumors, making it a target for cancer therapies (22). Specifically, research has shown that interfering with Activin A activity can assist in overcoming CD8 T-cell exclusion and immunotherapy resistance (23). In bone marrow-derived stem cell transplants for treatment of diabetes, Activin A enhances migration and homing of stem cells towards pancreatic lineage (24).
    References
    1. Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786.
    2. Maeshima, A. et al. (2008) Endocr. J. 55:1.
    3. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123.
    4. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
    5. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303.
    6. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
    7. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534.
    8. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
    9. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
    10. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
    11. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73.
    12. Onichtchouk, D. et al. (1999) Nature 401:480.
    13. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254.
    14. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490.
    15. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65.
    16. Schneyer, A. et al. (2003) Endocrinology 144:1671.
    17. Ghorbani-Dalini, S. et al. (2020) 3 Biotech. 10:215.
    18. Mennen, R. H. et al. (2022) Reprod Toxicol. 107:44.
    19. Mishra, S. et al. (2021) Stem Cells. 39:551.
    20. Tanigawa, S. et al. (2019) Stem Cell Reports 13:322.
    21. Cangkrama, M. et al. (2020) Trends Mol. Med. 26:1107.
    22. Ries, A. et al. (2020) Expert Opin. Ther. Targets. 24:985.
    23. Pinjusic, K. et al. (2022) J. Immunother. Cancer. 10:e004533.
    24. Dadheech, N. et al. (2020) Stem Cell Res. Ther. 11:327. 
    Entrez Gene IDs
    3624 (Human); 16323 (Mouse); 29200 (Rat)
    Alternate Names
    Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in PBS.

    参考图片

    Recombinant Human Latent Activin A (Catalog #
    9129-LA) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 is typically
    0.6-3.6 ng/mL.

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    9129-LA-025
    一键复制
    询价
    25ug
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价