首页
rmGalectin-2, CF (50 ug)
rmGalectin-2, CF (50 ug)

rmGalectin-2, CF (50 ug)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    产品介绍
    产品信息

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    8579-GA

    FormulationLyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
    ReconstitutionReconstitute at 400 μg/mL in water.
    ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Mouse Galectin-2 Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE with silver staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells. The ED50 for this effect is 0.5-2.5 μg/mL. Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 2.5-12.5 μg/mL.
    Source
    Human embryonic kidney cell, HEK293-derived mouse Galectin-2 protein
    Ser2-Glu130
    Accession #
    AAH27504
    N-terminal Sequence
    Analysis
    Ser2
    Predicted Molecular Mass
    15 kDa
    SDS-PAGE
    12-14 kDa, reducing conditions
    背景
    别名
    Beta-galactoside-binding lectin L-14-II,GAL2,Gal-2,galectin 2,Galectin2,Galectin-2,HL14gal-2,Lactose-binding lectin 2,lectin, galactoside-binding, soluble, 2,LGALS2,MGC75071,S-Lac lectin 2
    背景

    Background: Galectin-2

    Galectin-2 is an approximately 14 kDa, homodimeric carbohydrate-binding protein of the S-type lectin family (1, 2). Like other prototype Galectins, Galectin-2 consists of a single carbohydrate-recognition domain (CRD) (3). Mouse Galectin-2 shares 86% and 66% amino acid sequence identity with rat and human Galectin-2, respectively. Galectin-2 is primarily expressed in the digestive tract and skin of mice (4-6). It can be S-nitrosylated with S-nitrosoglutathione in mouse gastric mucosa (7).
    S-nitrosylation prevents Galectin-2 inactivation by hydrogen peroxide, suggesting that this modification protects Galectin-2 function against oxidative stress (8). Galectin-2 appears to have a role in immune responses. Galectin-2 mRNA and protein levels are down-regulated following infection with H. felis (9). Additionally, Galectin-2 treatment has been shown to reduce MHC class I-restricted immune responses and ameliorate acute and chronic experimental colitis in mice by inducing apoptosis of T cells (6, 10). Galectin-2 may also play a role in cancer since circulating levels of human Galectin-2 are elevated in colon and breast cancer patients (11).

    References
    1. Hokama, A. et al. (2008) World J. Gastroenterol. 14:5133.
    2. Barondes, S.H. et al. (1994) Cell 76:597.
    3. Hirabayashi, J. and K. Kasai (1993) Glycobiology 3:297.
    4. Nio, J. et al. (2005) J. Histochem. Cytochem. 53:1323.
    5. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
    6. Loser, K. et al. (2009) J. Immunol. 182:5419.
    7. Ohtake, K. et al. (2009) J. Proteomics 72:750.
    8. Tamura, M. et al. (2015) Biochem. Biophys. Res. Commun. 457:712.
    9. Takaishi, S. and T.C. Wang (2007) Cancer Sci. 98:284.
    10. Paclik, D. et al. (2008) J. Mol. Med. 86:1395.
    11. Chen, C. et al. (2014) Br. J. Cancer 110:741.
    Entrez Gene IDs
    3957 (Human); 107753 (Mouse); 171134 (Rat)
    Alternate Names
    Beta-galactoside-binding lectin L-14-II; GAL2; Gal-2; galectin 2; Galectin2; Galectin-2; HL14gal-2; Lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; LGALS2; MGC75071; S-Lac lectin 2
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 400 μg/mL in water.
    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    8579-GA-050
    一键复制
    询价
    50ug
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价