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品牌: BD Pharmingen
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咨询实验应用:
Flow cytometry, Immunocytochemistry, Immunofluorescence, Intracellular staining (flow cytometry) (Tested During Development)
产品介绍
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产品信息
简单描述
BD Via-Probe™ Red Nucleic Acid Stain is a nucleic acid dye that is useful for the discrimination of viable from nonviable cells, DNA content analysis in fixed cells, or nuclear counterstaining for fixed cells in immunofluorescence or flow cytometry applications. Although this dye is impermeant to viable cells with intact plasma membranes, it brightly stains nonviable or fixed cells with permeable membranes. BD Via-Probe™ Red Nucleic Acid Stain has an excitation maximum of 631 nm, and is best excited by the red laser. BD Via-Probe™ Red Nucleic Acid Stain has an emission maximum of 651 nm.

商品描述
BD Via-Probe™ Red Nucleic Acid Stain is a nucleic acid dye that is useful for the discrimination of viable from nonviable cells, DNA content analysis in fixed cells, or nuclear counterstaining for fixed cells in immunofluorescence or flow cytometry applications. Although this dye is impermeant to viable cells with intact plasma membranes, it brightly stains nonviable or fixed cells with permeable membranes. BD Via-Probe™ Red Nucleic Acid Stain has an excitation maximum of 631 nm, and is best excited by the red laser. BD Via-Probe™ Red Nucleic Acid Stain has an emission maximum of 651 nm.

浓度
5 mM

BD化合物表
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应用
实验应用
Flow cytometry, Immunocytochemistry, Immunofluorescence, Intracellular staining (flow cytometry) (Tested During Development)

目标/特异性
DNA Dyes

文献
文献
研发参考(4)
1. Darzynkiewicz Z, Bruno S, Del Bino G, et al. Features of apoptotic cells measured by flow cytometry. Cytometry. 1992; 13(8):795-808. (Methodology: Immunocytochemistry).
2. Darzynkiewicz Z, Halicka HD, Zhao H. Analysis of cellular DNA content by flow and laser scanning cytometry.. Adv Exp Med Biol. 2010; 676:137-47. (Methodology).
3. Hotz MA, Gong J, Traganos F, and Darzynkiewicz Z. Flow cytometric detection of apoptosis: Comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry. 1994; 15(3):237-244. (Methodology).
4. Schimenti KJ, Jacobberger JW. Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence.. Cytometry. 1992; 13(1):48-59. (Methodology).

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