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产品介绍
产品介绍
产品信息
荧光素标记
宿主
Mouse BALB/c IgG1, κ
免疫原
Human Peripheral Blood T Cells
简单描述
The CD8 antibody, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.
The CD8 antibody recognizes the 32-kilodalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (Mr 32, 30 kDa), while CD8+CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ+ T lymphocytes express an α/α homodimer (Mr 30 kDa). CD8+TCR-α/β+ T lymphocytes can express either an α/α homodimer or α/β heterodimer. The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex.
商品描述
SK1
The CD8 antibody, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.
The CD8 antibody recognizes the 32-kilodalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (Mr 32, 30 kDa), while CD8+CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ+ T lymphocytes express an α/α homodimer (Mr 30 kDa). CD8+TCR-α/β+ T lymphocytes can express either an α/α homodimer or α/β heterodimer. The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 SK1 (also known as Leu-2a; Leu2a) (RUO (GMP))
浓度
5.0 μg/mL
产品详情
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
482 nm
676 nm
BD化合物表
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应用
实验应用
Flow cytometry
推荐用量
20 μL
反应种属
Human
目标/特异性
CD8
背景
别名
CD8α; CD8A; CD8 alpha; Leu2a; MAL; T8; p32
制备和贮存
存储溶液
Phosphate buffered saline with gelatin and 0.1% sodium azide.
数据库链接
Entrez-Gene ID
925
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