Rhodopsin (RHO, Opsin-2, OPN2, OPSD, Opsin 2 Rod Pigment, MGC138309, Retinitis Pigmentosa 4, RHO 4D2)

Bovine

Mouse

Bovine Rhodopsin

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

IgG1

1D4

Monoclonal

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.

E IC IHC IP WB

Mouse

Bo Bu Ca Dk Eq Fe Gt Hu Ma Mk Mo Po Rb Rt Rd Sh

Recognizes bovine Rhodopsin at ~40kD. Species Crossreactivity: vertebrates (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the N-terminal of Rhodopsin.

RHO, Opsin-2, OPN2, OPSD, Opsin 2 Rod Pigment, MGC138309, Retinitis Pigmentosa 4, RHO 4D2

P08100

P08100