BD Transduction Laboratories™ Purified Mouse Anti-RIP

RIP

Mouse IgG2a

Human RIP aa. 385-650

Binding or cross linking of the Fas antigen (also known as APO-1 and CD95) is known to elicit apoptosis in susceptible cells. Fas is a member of a family of cell surface receptors which includes tumor necrosis factor receptors (TNF-R, and TNF-R2) and nerve growth factor receptors (NGF-R), CD40, OX40, CD30, CD27, and 4-1BB. Several members of this family have been shown to regulate or induce cell death (TNF-R1 and TNF-R2). A 74 kDa member of this family protein named RIP (Receptor Interacting Protein) contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic "death domain" present in both Fas and TNF-R1. Both Fas and RIP have been shown to require this death domain to induce apoptosis and overexpression of RIP has been shown to induce cell death in transfected cells.

38/RIP Binding or cross linking of the Fas antigen (also known as APO-1 and CD95) is known to elicit apoptosis in susceptible cells. Fas is a member of a family of cell surface receptors which includes tumor necrosis factor receptors (TNF-R, and TNF-R2) and nerve growth factor receptors (NGF-R), CD40, OX40, CD30, CD27, and 4-1BB. Several members of this family have been shown to regulate or induce cell death (TNF-R1 and TNF-R2). A 74 kDa member of this family protein named RIP (Receptor Interacting Protein) contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic "death domain" present in both Fas and TNF-R1. Both Fas and RIP have been shown to require this death domain to induce apoptosis and overexpression of RIP has been shown to induce cell death in transfected cells.

Mouse IgG2a

克隆 38/RIP (RUO)

250 µg/ml

Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)

Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)

RIP

Receptor Interacting Protein

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

研发参考(5) 1. Devin A, Lin Y, Yamaoka S, Li Z, Karin M, Liu Zg. The alpha and beta subunits of IkappaB kinase (IKK) mediate TRAF2-dependent IKK recruitment to tumor necrosis factor (TNF) receptor 1 in response to TNF. Mol Cell Biol. 2002; 21(12):3986-3994. (Biology: Western blot). 2. Fulda S, Meyer E, Debatin KM. Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression. Cancer Res. 2000; 60(14):3947-3956. (Biology: Western blot). 3. Lewis J, Devin A, Miller A, et al. Disruption of hsp90 function results in degradation of the death domain kinase, receptor-interacting protein (RIP), and blockage of tumor necrosis factor-induced nuclear factor-kappaB activation. J Biol Chem. 2000; 275(14):10519-10526. (Biology: Immunoprecipitation, Western blot). 4. Stanger BZ, Leder P, Lee TH, Kim E, Seed B. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell. 1995; 81(4):513-523. (Biology). 5. Takahashi T, Tanaka M, Brannan CI, et al. Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand. Cell. 1994; 76(6):969-976. (Biology).