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RIP3 (E7A7F) XP ® Rabbit mAb

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RIP3 (E7A7F) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

RIP3

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RIP3 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:50 - 1:200
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:400 - 1:1600

同种型

Rabbit IgG

分子量

46-62

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

RIP3 (E7A7F) XP Rabbit mAb recognizes endogenous levels of total RIP3 protein.

Species Reactivity:

Human

敏感性

Endogenous

背景

The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-κB, as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD (2,3). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (4,5). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP (8).Receptor-interacting protein 3 (RIP3) was originally found to interact with RIP and the TNF receptor complex to induce apoptosis and activation of NF-κB (9,10). It has subsequently been shown that the association between RIP and RIP3 is a key component of a signaling pathway that results in programmed necrosis (necroptosis), a necrotic-like cell death induced by TNF in the presence of caspase inhibitors (11-13). RIP3 is phosphorylated at Ser227 and targets the phosphorylation of mixed lineage kinase domain-like protein (MLKL), which is critical for necroptosis (14). 1.Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9. 2.Hsu, H. et al. (1996) Immunity 4, 387-96. 3.Stanger, B.Z. et al. (1995) Cell 81, 513-23. 4.Ting, A.T. et al. (1996) EMBO J 15, 6189-96. 5.Kelliher, M.A. et al. (1998) Immunity 8, 297-303. 6.Devin, A. et al. (2000) Immunity 12, 419-29. 7.Zhang, S.Q. et al. (2000) Immunity 12, 301-11. 8.Lin, Y. et al. (1999) Genes Dev 13, 2514-26. 9.Yu, P.W. et al. (1999) Curr Biol 9, 539-42. 10.Sun, X. et al. (1999) J Biol Chem 274, 16871-5. 11.Zhang, D.W. et al. (2009) Science 325, 332-6. 12.He, S. et al. (2009) Cell 137, 1100-11. 13.Cho, Y.S. et al. (2009) Cell 137, 1112-23. 14.Sun, L. et al. (2012) Cell 148, 213-27.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

11035

UniProt ID

Q9Y572

参考图片

Flow cytometric analysis of SU-DHL-4 cells (blue, negative) and THP-1 cells (green, positive) using RIP3 (E7A7F) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from various cell lines using RIP3 (E7A7F) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Absence of signal in HeLa cells is predicted from published reports of epigenetic loss of expression and confirms the specificity of the antibody for RIP3.

Immunoprecipitation of RIP3 protein from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is RIP3 (E7A7F) XP® Rabbit mAb. Western blot analysis was performed using RIP3 (E7A7F) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), hepatocellular carcinoma (middle), or normal thymus (right) using RIP3 (E7A7F) XP® Rabbit mAb (top) or RIP3 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human RIP3 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using RIP3 (E7A7F) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded HT-29 cell pellet (left, high-expressing) or HeLa cell pellet (right, low-expressing) using RIP3 (E7A7F) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human colon using RIP3 (E7A7F) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using RIP3 (E7A7F) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using RIP3 (E7A7F) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using RIP3 (E7A7F) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using RIP3 (E7A7F) XP® Rabbit mAb.

Confocal immunofluorescent analysis of HT-29 cells (left, positive) or HeLa cells (right, negative) using RIP3 (E7A7F) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

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货号：

10188S

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