RNeasy MinElute Cleanup Kit (50)

品牌： Qiagen

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50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers

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Sample Tech Manual Consumables

Product Details

The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silica-membrane technology. The kit can also be used to desalt RNA samples. Up to 45 µg RNA can be purified in a volume as low as 10 µl. Purification can be fully automated on the QIAcube Connect.

Performance

The RNeasy MinElute Cleanup Kit provides high-quality total RNA, free from impurities or enzymatic inhibitors, with A₂₆₀/A₂₈₀ ratios of 1.9–2.1 (see figure ""). RNA amounts corresponding to less than one cell (as little as 1 pg) can be concentrated (see figure ""). A large amount of RNA (up to 45 µg) can be purified and is suitable for use in sensitive assays (see figure ""). Reaction volumes can be kept small in downstream applications, giving increased reaction efficiency. The high purity RNA allows more of the sample to be used in reactions without inhibition (see figure "").

See figures

High-quality RNA.

Concentration of RNA.

Reliable cleanup of RNA.

Efficient removal of real-time RT-PCR inhibitors.

Principle

The RNeasy MinElute Cleanup Kit is designed to purify and concentrate RNA from enzymatic reactions (e.g., labeling, in vitro transcription), RNA isolated by alcohol-precipitation and organic-extraction methods, as well as to desalt RNA samples, and concentrate RNA prepared by silica-membrane procedures (e.g., PAXgene Blood RNA preps). RNeasy technology simplifies total RNA isolation by combining guanidine-isothiocyanate lysis with silica-membrane purification.

Procedure

Guanidine-isothiocyanate–containing lysis buffer and ethanol are added to the sample to create conditions that promote selective binding of RNA to the RNeasy MinElute membrane. The sample is then applied to the RNeasy MinElute spin column. RNA binds to the silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in water.

Enzymatic reactions or crude RNA preps are simultaneously cleaned up and concentrated in less than 15 minutes. In comparison, time-consuming concentration and cleanup by alcohol precipitation can result in loss of RNA, especially from small samples. The RNeasy MinElute procedure is faster than vacuum centrifugation, which only concentrates the sample without removing salts and other impurities. The unique design of RNeasy MinElute spin columns enables concentration of purified RNA to as little as 10 µl for downstream applications such as microarray analysis and real-time RT-PCR. Purification can be fully automated on the QIAcube Connect.

Applications

RNA purified with RNeasy MinElute technology is high-quality and ideal for use in all applications including:

Northern, dot, and slot blotting
End-point RT-PCR
Quantitative, real-time RT-PCR
Array analysis
Poly A⁺ RNA selection

Supporting data and figures

High-quality RNA.

Total RNA was isolated from HeLa cells using an acid-phenol method plus cleanup and concentration using the RNeasy MinElute Cleanup Kit. The high quality of the RNA is shown by scanning with the Agilent 2100 Bioanalyzer.

Specifications

Features	Specifications
Applications	PCR, qPCR, real-time PCR, microarray
Format	MinElute Spin column
Sample amount	200 µl
Elution volume	10–14 µl
Processing	Manual
Main sample type	(Crude) RNA preps
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Time per run or per prep	<15 minutes
Technology	Silica technology
Yield	45 µg

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货号：

74204

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