Rabbit Polyclonal ROBO1 Antibody

Immunogen affinity purified

Rabbit

This affinity-purified ROBO1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO1. (Uniprot: Q2M1J3)

This affinity purified antibody is directed against human ROBO1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Polyclonal

Immunogen affinity purified

0.01% Sodium Azide

Polyclonal

ROBO1

ELISA 1:30000-1:60000Immunocytochemistry/ Immunofluorescence 1:10-1:500Immunohistochemistry 2 ug/ml-10 ug/mlImmunohistochemistry-Paraffin 1:10-1:500Immunoprecipitation 1:10 - 1:500Western Blot 1:500-1:3000

Hu, MuSpecies Glossary

This affinity purified antibody is directed against human ROBO1 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO1. Cross reactivity with ROBO1 homologues from other sources has not been determined.

20mM Sodium Phosphate (pH 7.2) and 0.15M NaCl