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BD Pharmingen™ PE Mouse anti-Mouse RORγt

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BD Pharmingen™ PE Mouse anti-Mouse RORγt

品牌： BD Pharmingen

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产品介绍

产品信息

荧光素标记

抗原名称

RORγt

宿主

Mouse IgG2a, κ

免疫原

Mouse RORγt Recombinant Protein

简单描述

The Q31-378 monoclonal antibody recognizes mouse RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.

商品描述

Q31-378 The Q31-378 monoclonal antibody recognizes mouse RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.

同种型

Mouse IgG2a, κ

克隆号

克隆 Q31-378 (RUO)

浓度

0.2 mg/ml

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Intracellular staining (flow cytometry) (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

RORγt

背景

别名

RORγT; RORgt; RORgamma t; RORgammaT; Rorc2; Rorg; TOR; Thor; Nr1f3

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(9) 1. Bechtel S, Rosenfelder H, Duda A, et al. The full-ORF clone resource of the German cDNA Consortium. BMC Biochem. 2007; 8:399-410. (Biology). 2. Eberl G, Littman DR. The role of the nuclear hormone receptor RORgt in the development of lymph nodes and Peyer's patches. Immunol Rev. 2003; 195(195):81-90. (Biology). 3. Eberl G, Marmon S, Sunshine MJ, Rennert PD, Choi Y, Littman DR. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol. 2004; 5(1):64-73. (Biology). 4. Hamada H, Garcia-Hernandez MdlL, Reome JB, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009; 182(6):3469-3481. (Biology). 5. He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells. Immunity. 1998; 9(6):797-806. (Biology). 6. Hirose T, Smith RJ, Jetten AM. ROR gamma: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle. Biochim Biophys Acta. 1994; 205(3):1976-1983. (Biology). 7. Ivanov, II, McKenzie BS, Zhou L, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006; 126(6):1121-1133. (Biology). 8. Medvedev A, Yan ZH, Hirose T, Giguere V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996; 181(1-2):199-206. (Biology). 9. Unutmaz D. RORC2: the master of human Th17 cell programming. Eur J Immunol. 2009; 39(6):1452-1455. (Biology).

参考图片

Multicolor flow cytometric analysis of mouse RORγt expression in thymocytes and Th17 polarized cells. BALB/c mouse thymocytes (Panel 1) or Th17 polarized cells (Panels 2 and 3) were stained with BD Horizon™ Fixable Viability Stain 450 (Cat. No. 562247), fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574) and then stained with either PE Mouse Anti-Mouse RORγt antibody (Cat. No. 562607) or PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648). The thymocytes were counterstained with APC Rat Anti-Mouse CD4 (Cat. No. 553051) and PerCP-Cy™5.5 Rat Anti-Mouse CD8a (Cat. No. 551162) antibodies. The Th17-polarized cells were counterstained with APC-Cy™7 Hamster Anti-Mouse CD3e (Cat. No. 557596), Alexa Fluor® 700 Rat Anti-Mouse CD4 (Cat. No. 557956), FITC Rat Anti-Mouse IFN-γ (Cat. No. 554411) and PerCP-Cy™5.5 Rat Anti-Mouse IL-17A (Cat. No. 560666) antibodies. Panel 1: The overlapping histograms show the levels of IgG2a Control (Left) and RORγt (Right) staining in CD4+CD8- single-positive (dotted line) or CD4+CD8+ double-positive (solid line) thymocytes. Panel 2: The overlapping histograms show the levels of IgG2a Control (Left) and RORγt (Right) staining in Th17-polarized IL-17A-IFN-γ+ (dotted line) or IL-17A+IFN-γ- (solid line) cells. Panel 3: Two-color contour plots show the correlated expression patterns of IgG2a Control (Left) or ROR γ t (Right) staining versus IL-17A in Th17-polarized CD3+CD4+ cells. The flow cytometric data were derived from viable cell-discriminated events with the forward and side light-scatter characteristics of intact single cells using a BD LSRFortessa™ Cytometer System.

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货号：

562607

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