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Ser21-Gln265, with C-terminal His Tag
27-30kDa (Reducing)
1μg (R: reducing condition, N: non-reducing condition).
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27-30 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members. RSPO1 is a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids.
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
1.Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
2.Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
3.Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
4.Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
5.Parma, P. et al. (2006) Nat. Genet. 38:1304.
参考图片
1μg (R: reducing condition, N: non-reducing condition).
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical EC50 is less than 40ng/ml in the presence of 5ng/ml Recombinant Human Wnt3a.