RSPO1 Protein, Mouse

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RSPO1 Protein, Mouse

品牌： UA BIOSCIENCE

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参数

分子量：

27-30kDa (Reducing)

纯度：

＞95% by SDS-PAGE&RP-HPLC

产品介绍

产品信息

氨基酸序列

Ser21-Gln265, with C-terminal His Tag

分子量

27-30kDa (Reducing)

生物活性

＜40ng/ml

纯度

＞95% by SDS-PAGE&RP-HPLC

内毒素水平

<0.1EU/μg

性状

Lyophilized Powder

表达系统

CHO

缓冲体系

PBS, 5% trehalose, PH7.4

标签

His Tag

种属

Mouse

电泳JSON

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1μg (R: reducing condition, N: non-reducing condition).

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背景

别名

R-spondin1, Cristin-3, mCristin-3

背景

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27-30 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members. RSPO1 is a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids.

制备和贮存

溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

保存方式

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.

文献

1.Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.

2.Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.

3.Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.

4.Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.

5.Parma, P. et al. (2006) Nat. Genet. 38:1304.

数据库链接

Accession

Q9Z132

参考图片

1μg (R: reducing condition, N: non-reducing condition).

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical EC50 is less than 40ng/ml in the presence of 5ng/ml Recombinant Human Wnt3a.

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货号：

UA040213-1mg

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