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Western Blot: SLC36A4 Antibody (PAT-4/9/H10) [NBP2-50237] - Analysis of serial dilutions of cell lysates (15, 7.5 and 3.8?µg of protein) produced from pools of 786-O cells carrying an IPTG-inducible shPAT4 (43587; shPAT4) probed with PAT4 monoclonal antibody Pat/9/H10. This reveals a set of bands from 60–75kDa that is strongly reduced by IPTG-induced PAT4 knockdown (+IPTG), suggesting that they are PAT4-specific. Western blots were also probed with an anti-tubulin antibody as a loading control. (from Fan et al. 2015. Oncogene. :. PMID: 26434594.)
Immunohistochemistry: SLC36A4 Antibody (PAT-4/9/H10) [NBP2-50237] - Staining of 786-O cells and visualised with 3,3'-diaminobenzidine (DAB). There is an obvious perinuclear region of staining visible in most cells treated with the scrambled control siRNA (a). This staining is absent when the PAT4 transcript is knocked down using an siRNA against PAT4 (b). (from Fan et al. 2015. Oncogene. :. PMID: 26434594.)