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品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing), Mouse,Rat (Predicted)
来源宿主：
Mouse BALB/c IgG1, κ
产品介绍
产品介绍
产品信息
抗原名称
S6 (pS235/pS236)
宿主
Mouse BALB/c IgG1, κ
免疫原
Phosphorylated Human ribosomal protein S6 Peptide
简单描述
Ribosomal protein S6 (
~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells.
The N7-548 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S235 and S236.
商品描述
N7-548
Ribosomal protein S6 (
~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells.
The N7-548 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S235 and S236.
同种型
Mouse BALB/c IgG1, κ
克隆号
克隆 N7-548 (RUO)
产品详情
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
应用
实验应用
Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging (Not Recommended)
推荐用量
20 µl
反应种属
Human (QC Testing), Mouse,Rat (Predicted)
目标/特异性
S6 (pS235/pS236)
背景
别名
40S ribosomal protein S6; Phosphoprotein NP33; RPS6; RS6
翻译后修饰
Phosphate
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(9)
1. Blatt K, Herrmann H, Mirkina I, et al. The PI3-kinase/mTOR-targeting drug NVP-BEZ235 inhibits growth and IgE-dependent activation of human mast cells and basophils. PLoS ONE. 2012; 7(1):e29925. (Clone-specific: Flow cytometry).
2. Corradetti MN, Inoki K, Guan KL. The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J Biol Chem. 2005; 280(11):9769-9772. (Biology).
3. Gu JJ, Santiago L, Mitchell BS. Synergy between imatinib and mycophenolic acid in inducing apoptosis in cell lines expressing Bcr-Abl. Blood. 2005; 105(8):3270-3277. (Biology).
4. Jastrzebski K, Hannan KM, Tchoubrieva EB, Hannan RD, Pearson RB. Coordinate regulation of ribosome biogenesis and function by the ribosomal protein S6 kinase, a key mediator of mTOR function. Growth Factors. 2007; 25(4):209-226. (Biology).
5. Kleijn M, Proud CG. The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes. BMC Biochem. 2002; 3:11. (Biology).
6. Lal L, Li Y, Smith J, et al. Activation of the p70 S6 kinase by all-trans-retinoic acid in acute promyelocytic leukemia cells.. Blood. 2005; 105(4):1669-77. (Biology).
7. Shah OJ, Ghosh S, Hunter T. Mitotic regulation of ribosomal S6 kinase 1 involves Ser/Thr, Pro phosphorylation of consensus and non-consensus sites by Cdc2. J Biol Chem. 2003; 278(18):16433-16442. (Biology).
8. Shah OJ, Hunter T. Critical role of T-loop and H-motif phosphorylation in the regulation of S6 kinase 1 by the tuberous sclerosis complex. J Biol Chem. 2004; 279(20):20816-20823. (Biology).
9. van de Laar L, van den Bosch A, Boonstra A, et al. PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function. Blood. 2012; 120(25):4982-4991. (Clone-specific: Flow cytometry).
参考图片
Analysis of S6 (pS235/pS236) in activated human peripheral blood mononuclear cells (PBMC). PBMC were isolated by density gradient centrifugation (Ficoll-Paque™ PLUS, Cat. No. 17-1440-02) and either left untreated (open histogram) or treated with PMA (Sigma-Aldrich, Cat. No. P8139) at 50 nM/10^6 cells for 30 minutes (shaded histogram). Cells were then fixed in BD Cytofix™ buffer (Cat. No. 554655) at 37°C for 10 minutes, then permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 488 Mouse anti-S6 (pS235/pS236). For data analysis, lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Western blot analysis of S6 (pS235/pS236). The specificity of mAb N7-548 was confirmed by western blot analysis using unconjugated Mouse anti-S6 (pS235/pS236) antibody on lysates from untreated (lane 1) or PMA-treated (lane 2) PBMC. S6 (pS235/pS236) is identified as a band of 32 kDa, with increased intensity in the PMA-treated cells. Purified Mouse anti-Actin monoclonal antibody (Cat. No. 612656 or 612657) was the gel-loading control.
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