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rmSCF, CF (10 ug)
rmSCF, CF (10 ug)
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rmSCF, CF (10 ug)

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    Scientific Data

    Bioactivity Recombinant Mouse SCF Protein BioactivityView Larger

    Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 2.5-10 ng/mL.

    SDS-PAGE Recombinant Mouse SCF Protein SDS-PAGEView Larger

    1 μg/lane of Recombinant Mouse SCF/c-kit was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    455-MC

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    455-MC/CF

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Mouse SCF Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 2.5-10 ng/mL.
    Source
    E. coli-derived mouse SCF/c-kit Ligand protein
    Lys26-Ala189, with an N-terminal Met
    Accession #
    Q78ED8
    N-terminal Sequence
    Analysis
    Met
    Predicted Molecular Mass
    18.6 kDa
    背景
    背景

    Background: SCF/c-kit Ligand

    Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

    References
    1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
    2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
    3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
    4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
    5. Kapur, R. et al. (2002) Blood 100:1287.
    6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
    7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
    8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
    9. Huang, E. et al. (1990) Cell 63:225.
    10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
    11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
    12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
    13. Flanagan, J.G. et al. (1991) Cell 64:1025.
    14. Martin, F.H. et al. (1990) Cell 63:203.
    15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
    16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
    Long Name
    Stem Cell Factor
    Entrez Gene IDs
    4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
    Alternate Names
    c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is typically 2.5-10 ng/mL.

    1 μg/lane of Recombinant Mouse SCF/c-kit was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

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    货号：
    455-MC-010/CF
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