Monkey SCF ELISA Kit

stem cell factor, partial

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of MonkeyMAdCAM1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MonkeyMAdCAM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MonkeyMAdCAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MonkeyMAdCAM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MonkeyMAdCAM1. You can calculate the concentration of MonkeyMAdCAM1 in the samples by comparing the OD of the samples to the standard curve.

微量ELISA板（免疫球蛋白抗体预包被96孔板）；标准品（重组干细胞因子蛋白）；浓缩生物素检测抗体；浓缩HRP结合物（HRP酶标记的链霉亲和素）；标准品样品稀释剂（磷酸盐缓冲液）；生物素检测抗体稀释剂（磷酸盐缓冲液）；HRP结合物稀释剂（三(羟甲基)氨基甲烷溶液）；浓缩洗涤缓冲液25X（0.05%吐温20、99.95%水）；TMB基质（四甲基联苯胺溶液）；终止液（20%氢氧化钠溶液）；粘合板密封件

Monkey

0.16110ng/mL

Quantitative Sandwich

Serum, Plasma And Other Biological Fluids

0.09ng/mL.