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SDHA (D6J9M) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

抗原名称

SDHA

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly166 of human SDHA protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying SDHA. Cited in 115 publications. Validated for WB, IP, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SDHA (D6J9M) XP® Rabbit Monoclonal Antibody (CST #11998) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:50 - 1:200

分子量

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Hamster,Monkey,

目标/特异性

Specificity/Sensitivity

SDHA (D6J9M) XP® Rabbit mAb recognizes endogenous levels of total SDHA protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

敏感性

Endogenous

背景

Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington’s disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas (4,5). 1.Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31. 2.Bourgeron, T. et al. (1995) Nat Genet 11, 144-9. 3.Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63. 4.Baysal, B.E. et al. (2000) Science 287, 848-51. 5.Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

6389

UniProt ID

P31040

参考图片

Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析293细胞，使用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗（绿色）和β-Actin (8H10D10) Mouse mAb鼠单抗#3700（红色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。

Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.

Wetern blot检测多种细胞系提取物，所用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗。

Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.

免疫沉淀方法检测HeLa细胞提取物SDHA蛋白，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或SDHA (D6J9M) XP® Rabbit mAb兔单抗 (泳道3)。泳道1为10%对照。使用SDHA (D6J9M) XP® Rabbit mAb兔单抗进行Western blot分析。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗，对照（左）或抗原特异性肽（右）。

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货号：

11998S

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20ul

100ul

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