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DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)
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咨询Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide.
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
| Chromatin IP | 1:50 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #70586.
参考图片
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells, untransfected (left) or transfected (right) with a DYKDDDDK-tagged SSRP1 construct and DYKDDDDK (D6W5B) Rabbit mAb, SSRP1 (E1Y8D) Rabbit mAb #13421, or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Exon 3 Primers #12010 and SimpleChIP® Human EGR1 Intron 3 Primers #11953. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with DYKDDDDK-GFP (N-terminal DDK-Tag; +), GFP-DYKDDDDK (C-terminal DDK-Tag; +), human CASQ1-DYKDDDDK (C-terminal DDK-Tag; +), or human FoxG1-DYKDDDDK (C-terminal DDK-Tag; +) as indicated, using DYKDDDDK Tag (D6W5B) Rabbit mAb.
Western blot analysis of recombinant human SYN3-MYC/DYKDDDDK at various concentrations using, DYKDDDDK Tag (D6W5B) Rabbit mAb.
Immunoprecipitation of FoxG1-DYKDDDDK tag protein from 293T transfected cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DYKDDDDK (D6W5B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DYKDDDDK (D6W5B) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or DYKDDDDK Tag-transfected (right), using DYKDDDDK Tag (D6W5B) Rabbit mAb.
Confocal immunofluorescent analysis of 293T cells transfected with a GFP-DYKDDDDK-Tag (green) using DYKDDDDK Tag (D6W5B) Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of 293 cells, untransfected (blue) or transfected with DYKDDDDK-Tag (green), using DYKDDDDK Tag (D6W5B) Rabbit mAb. Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.