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品牌: CST









产品介绍
产品介绍
产品信息
产品详情
Cell Cycle / Checkpoint Control
简单描述
The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells. Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.

研究领域
代谢,神经科学,

组成成分
水69.55%,5-溴-4-氯-3-吲哚基-.beta.-D-吡喃半乳糖苷0.45%,磷酸三钠5%,六氰合亚铁酸钾三水合物15%,柠檬酸5%,铁氰化钾5%

背景
背景
Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent β-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
1.Goldstein, S. (1990) Science 249, 1129-33.
2.Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci., USA 85, 9086-90.
3.Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-7.
4.Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
5.Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-51.

研究领域
代谢,神经科学,
翻译后修饰
unmodified

数据库链接
Entrez-Gene ID
945006

UniProt ID
P00722

参考图片
β-Galactosidase staining at pH 6 on normal WI38 cells at population doubling 29 (left) and senescent WI38 cells at population doubling 36 (right).
Β-Galactosidase staining at pH 6.0 on MCF-7 cells untreated (left) and senescent MCF-7 cells treated with etoposide #2200 (12.5 μM, 24 hr) and allowed to recover for 4 days (right).
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