SensoLyte520 TEV Protease Assay 1kit

Tobacco Etch Virus (TEV)

E. coli

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal. The SensoLyte® TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directlyproportional to the TEV protease quantity/activity.

Component A: 5-FAM/QXL™ 520 TEV Substrate, Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component B: 5-FAM, Fluorescence Reference Standard, Ex/Em=490 nm/520 nm: 100 µM, 10 µL Component C: TEV Protease, Recombinant: 2 µG X 4 vials Component D: Assay buffer: 30 mL Component E: DTT, 1M: 30 µL

Fluorescent;FRET,Protease activity