SF Cell Line 4D X Kit S (32 RCT)

品牌： Lonza

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商品描述

For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTMX Unit (20 µL format).Do you need a quote right away? Add this product to your shopping cart, go to your cart and click on “Generate Quote”. Your quote will be available straight away to view, save online, and download in PDF format.

组成成分

磷酸盐缓冲液100%、纯水100%、盐酸盐缓冲液100%

Product Overview

The SF Cell Line 4D-Nucleofector^TM X Kit S is one of our three 4D-Nucleofector^TM Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette^TM Strip format when working with the 4D-Nucleofector^TM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

293-F (FreeStyle), 32D
A-20, A2058, A375, A431, A549, AGS, ARPE-19
BetaTC/6, BxPC-3
C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
EL4
FDC-P1
H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
IMR-32
J774A.1
K-562
KG-1
L-428, LNCaP
MDA-MB-453, MDBK, Molt-4, MV-4-11
Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
P19, P815, PC-12, PC-3
Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
T2, T84, TF-1
U266B1, U-937
Vero

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

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Benefits

High transfection efficiencies in difficult-to-transfect cell lines
Cells maintain functionality post transfection
Conditions are transferable between 4D-Nucleofector^TM System, 96-well Shuttle^TM System and 384-well Nucleofector^TM System
Transferability to 4D-Nucleofector^TM LV Unit

Applications

Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells
Medium throughput applications
Optimization of transfection conditions e.g. substrate titrations

Storage and Content

1 x 0.675 mL SF Cell Line Nucleofector^{^®}; Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFP^TM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette^{^®} Strips (20 μL)

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货号：

V4XC-2032

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询价

32RCT

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