全部商品分类
Phospho-Shc (Tyr239/240) Antibody
品牌： CST
下载产品说明书 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询分子量：
50, 55, 70
反应种属：
Human,Mouse,Rat,
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Shc
来源纯化
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
分子量
50, 55, 70
研究领域
癌症,细胞生物学,
应用
反应种属
Human,Mouse,Rat,
目标/特异性
Specificity/Sensitivity
Phospho-Shc (Tyr239/240) Antibody detects Shc only when phosphorylated at tyrosine 239/240. The antibody may cross-react with activated EGF receptor protein.
Species Reactivity:
Human, Mouse, Rat
敏感性
Endogenous
背景
背景
SHC-transforming protein 1 (SHC1) is an adaptor protein to targets of activated tyrosine kinases. SHC1 is ubiquitously expressed and the gene is expressed in 2 transcripts, resulting in 3 isoforms with distinct functions and subcellular locations (1). The phosphorylated p46 and p52 isoforms couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex. The third isoform, p66, acts as a downstream target of the tumor suppressor p53 and is thereby implicated in oxidative stress response, tumor progression, and cancer cell proliferation (2,3). SHC1 has been identified to have prognostic and diagnostic value in multiple cancers, making it a potential biomarker (4).
1.Terada, L.S. (2019) Small GTPases 10, 64-71.
2.Audero, E. et al. (2004) J Biol Chem 279, 13224-33.
3.Lebiedzinska-Arciszewska, M. et al. (2015) Eur J Clin Invest 45 Suppl 1, 25-31.
4.Chen, J. et al. (2022) Front Genet 13, 817118.
研究领域
癌症,细胞生物学,
翻译后修饰
phosphate
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
6464
UniProt ID
P29353
参考图片
Western blot analysis of extracts from HepG2 cells, untreated or EGF-treated (100 ng/ml) 18 hours of serum-starvation, and Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-Shc (Tyr239/240) Antibody (upper) or Shc antibody #2432 (lower).
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。