Purified Mouse Anti-SHC(30/SHC)

Shc

Mouse IgG1

Human SHC aa. 359-473

The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal  glycine/proline rich sequence.  These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway.  To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs.  Phosphorylated SHC forms a complex with the adaptor protein GRB2.  Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane.  Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange.  Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases.  The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.

The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal  glycine/proline rich sequence.  These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway.  To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs.  Phosphorylated SHC forms a complex with the adaptor protein GRB2.  Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane.  Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange.  Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases.  The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.

Mouse IgG1

30/SHC

250 µg/ml

Western blot (Routinely Tested) Immunofluorescence (Tested During Development)

Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

研发参考(5) 1. Imokawa G, Kobayasi T, Miyagishi M. Intracellular signaling mechanisms leading to synergistic effects of endothelin-1 and stem cell factor on proliferation of cultured human melanocytes. Cross-talk via trans-activation of the tyrosine kinase c-kit receptor. J Biol Chem. 2000; 275(43):33321-33328. (Biology: Immunoprecipitation, Western blot). 2. Kiely PA, Sant A, O'Connor R. RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death. J Biol Chem. 2002; 277(25):22581-22589. (Biology: Immunoprecipitation, Western blot). 3. Laser M, Willey CD, Jiang W. Integrin activation and focal complex formation in cardiac hypertrophy. J Cell Biol. 2000; 275(45):35624-35630. (Biology: Western blot). 4. Pelicci G, Lanfrancone L, Grignani F. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction. Cell. 1992; 70(1):93-104. (Biology). 5. Ugi S, Imamura T, Ricketts W, Olefsky JM. Protein phosphatase 2A forms a molecular complex with Shc and regulates Shc tyrosine phosphorylation and downstream mitogenic signaling. Mol Cell Biol. 2002; 22(7):2375-2387. (Biology: Immunoprecipitation, Western blot).