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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly451 of human SHMT2 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Immunohistochemical analysis of paraffin-embedded RH-30 cell pellet (left, high-expressing) or MDA-MB-231 cell pellet (right, low-expressing) using SHMT2 (E7F4Q) Rabbit mAb.
Western blot analysis of extracts from RH-30, MDA-MB-231, and HeLa cells using SHMT2 (E7F4Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of SHMT2 protein from RH-30 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SHMT2 (E7F4Q) Rabbit mAb. Western blot analysis was performed using SHMT2 (E7F4Q) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using SHMT2 (E7F4Q) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using SHMT2 (E7F4Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using SHMT2 (E7F4Q) Rabbit mAb (left) or SHMT2 (E8O8J) Rabbit mAb (IHC Specific) #37004 (right). These two antibodies detect independent, unique epitopes on human SHMT2. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.