首页
rhST6GALNAC1 (20 ug)
rhST6GALNAC1 (20 ug)

rhST6GALNAC1 (20 ug)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    产品介绍
    产品信息

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9154-GT

    FormulationSupplied as a 0.2 μm filtered solution in Tris and NaCl.
    ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 6 months from date of receipt, -20 to -70 °C as supplied.
    • 3 months, -20 to -70 °C under sterile conditions after opening.

    Recombinant Human ST6GALNAC1 Protein Summary

    Learn more about Fluorescent Glycan Labeling and Detection

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum. The specific activity is >90 pmol/min/μg, as measured under the described conditions.
    Source
    Human embryonic kidney cell, HEK293-derived human ST6 Sialyltransferase 1/ST6GALNAC1 protein
    Lys36-Asn600, with a C-terminal 6-His tag
    Accession #
    Q9NSC7
    N-terminal Sequence
    Analysis
    Lys36 & Thr73
    Predicted Molecular Mass
    65 kDa
    SDS-PAGE
    70-89 kDa, reducing conditions
    背景
    别名
    alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1,EC 2.4.99.3,GalNAc alpha-2, 6-sialyltransferase I, long form,GalNAc alpha-2,6-sialyltransferase I,sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) A,Sialyltransferase 7A,Siat7A,SIAT7-A,ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 1,ST6GalNAc I,ST6GALNAC1,ST6GalNAcI,ST6GalNAcIHSY11339,STYI,ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 1
    背景

    Background: ST6 Sialyltransferase 1/ST6GALNAC1

    Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion. Sialyltransferases are key enzymes that regulate the serum levels of sialic acid‑containing molecules. Alpha‑N‑acetylgalactosaminide alpha ‑2,6‑sialyltransferase 1, or ST6GALNAC1, is a type II membrane protein localized in the Golgi network and catalyzes 2,6‑sialylation of the Tn antigen (GalNAc‑O‑Ser/Thr) (1, 2). Sialyl‑Tn antigen is highly expressed in several human carcinomas and is associated with carcinoma aggressiveness and poor prognosis (3). Breast cancer cells transfected with ST6GALNAC1 synthesized the sialyl‑Tn antigen structure (4). The enzyme may also be used to synthesize a cancer vaccine (5). The activity of this enzyme has been measured using a phosphatase‑coupled assay (6).

    References
    1. Ikehara, Y, et al. (1999) Glycobiology 9:1213.
    2. Sewell, R. et al. (2006) J. Biol. Chem. 281:3586.
    3. Lima, L. et al. (2013) Br. J. Cancer 109:2106.
    4. Julien, S. et al. (2001) Glycoconj. J. 18:883.
    5. Julien, S. et al. (2009) Br. J. Cancer. 100:1746.
    6. Wu, Z.L. et al. (2011) Glycobiology 21:727.
    Long Name
    ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 1
    Entrez Gene IDs
    55808 (Human); 20445 (Mouse); 287920 (Rat)
    Alternate Names
    alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; EC 2.4.99.3; GalNAc alpha-2, 6-sialyltransferase I, long form; GalNAc alpha-2,6-sialyltransferase I; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) A; Sialyltransferase 7A; Siat7A; SIAT7-A; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 1; ST6GalNAc I; ST6GALNAC1; ST6GalNAcI; ST6GalNAcIHSY11339; STYI
    研究领域
    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    9154-GT-020
    一键复制
    询价
    20ug
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价