Hu Siglec-6 (CD327) BV421 767329 50ug

CD327 (Siglec-6)

Mouse IgG2a, κ

Human Siglec-6 Recombinant Protein

The 767329 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 6 (Siglec-6) which is also known as Obesity-binding protein 1 (OB-BP1), CD33 antigen-like 1 (CD33L1), or CD327. Siglec-6 (CD327) is a type I transmembrane glycoprotein that is encoded by SIGLEC6 which belongs to the Sialic acid-binding Ig-like lectins (Siglecs) superfamily. Siglec-6 (CD327) has one N-terminal Ig-like V-set domain followed by two Ig-like C2-type domains in its extracellular region. Its intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as a signaling lymphocyte activating molecule (SLAM)-like motif that can serve as a docking site for  SAP (SLAM-associated protein). This I-type lectin can also exist in a soluble form of the extracellular region that lacks the transmembrane and cytoplasmic regions. Siglec-6 (CD327) is expressed on placental trophoblasts and B cells and at low levels on neutrophils. It specifically recognizes sialyl Tn-containing molecules and binds to leptin and is involved in cellular interactions and signaling. The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific BlueTM conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue, and BD Horizon V450 cannot be used simultaneously.

The 767329 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 6 (Siglec-6) which is also known as Obesity-binding protein 1 (OB-BP1), CD33 antigen-like 1 (CD33L1), or CD327. Siglec-6 (CD327) is a type I transmembrane glycoprotein that is encoded by SIGLEC6 which belongs to the Sialic acid-binding Ig-like lectins (Siglecs) superfamily. Siglec-6 (CD327) has one N-terminal Ig-like V-set domain followed by two Ig-like C2-type domains in its extracellular region. Its intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as a signaling lymphocyte activating molecule (SLAM)-like motif that can serve as a docking site for  SAP (SLAM-associated protein). This I-type lectin can also exist in a soluble form of the extracellular region that lacks the transmembrane and cytoplasmic regions. Siglec-6 (CD327) is expressed on placental trophoblasts and B cells and at low levels on neutrophils. It specifically recognizes sialyl Tn-containing molecules and binds to leptin and is involved in cellular interactions and signaling. The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific BlueTM conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue, and BD Horizon V450 cannot be used simultaneously.

Human (Tested in Development)

SIGLEC6; CD33 antigen-like 1; CD33L1; CD33L; OBBP1; obesity-binding protein 1

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(3) 1. Botting RA, Bertram KM, Baharlou H, et al. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes.. J Leukoc Biol. 2017; 101(6):1393-1403. (Clone-specific: Flow cytometry). 2. Madge PD, Maggioni A, Pascolutti M, et al. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy.. Sci Rep. 2016; 6:36012. (Clone-specific: Flow cytometry). 3. Takei Y, Sasaki S, Fujiwara T, Takahashi E, Muto T, Nakamura Y. Molecular cloning of a novel gene similar to myeloid antigen CD33 and its specific expression in placenta.. Cytogenet Cell Genet. 1997; 78(3-4):295-300. (Biology).