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SignalSilence ®  Bcl-xL siRNA I

SignalSilence ® Bcl-xL siRNA I

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品牌: CST
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反应种属:
Human
产品介绍
产品介绍
产品信息
简单描述

SignalSilence® Bcl-xL siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bcl-xL expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

商品描述

Product Usage Information

CST recommends transfection with 100 nM Bcl-xL siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

研究领域
细胞生物学,纤维化,神经科学
应用
反应种属
Human
背景
背景
Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5). 1.Adams, J.M. and Cory, S. (1998) Science 281, 1322-6. 2.Minn, A.J. et al. (1999) EMBO J 18, 632-43. 3.Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9. 4.Fan, M. et al. (2000) J Biol Chem 275, 29980-5. 5.Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.
研究领域
细胞生物学,纤维化,神经科学
翻译后修饰
unmodified
制备和贮存
保存方式

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

数据库链接
Entrez-Gene ID
598
UniProt ID
Q07817

参考图片

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® BcL-xL siRNA I (+) or SignalSilence® Bcl-xL siRNA II #6363 (+), using Bcl-xL (54H6) Rabbit mAb #2764 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Bcl-xL (54H6) Rabbit mAb confirms silencing of Bcl-xL expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

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货号:
6362S
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询价
300ul
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