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咨询RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. SignalSilence® Control siRNA (Fluorescein Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is fluorescein-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.
Product Usage Information
CST recommends transfection with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) 24 to 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by transfection reagent manufacturer. Please feel free to contact CST with any questions on use.Each vial contains the equivalent of 20 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 µl per well.
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
参考图片
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. TransIT-TKO® Transfection Reagent from Mirus was used (Mirus catalog #MIR2114).
Western blot analysis of extracts from HeLa cells, either untransfected or transfected with NF-κB p65 siRNA #6261 or SignalSilence® Control siRNA (Fluorescein Conjugate), using NF-κB p65 Antibody #3034.