SignalStain Aqueous Mounting Medium

SignalStain® Aqueous Mounting Medium is for use in immunohistochemical assays. This product is a hardsetting, aqueous-based mounting medium used to preserve tissue or cell preparations. SignalStain® Aqueous Mounting Medium can be used when the reaction products are soluble in alcohol or organic solvents. This product is compatible with most peroxidase and alkaline phosphatase enzymatic substrates.

Product Usage Information

a. To prevent bubbles, do not shake the bottle. Carefully tip the bottle and dispense drops, using constant pressure on the bottle. If any bubbles are in the bottle tip, dispense and discard several drops before use. b. Add 1 or 2 drops of SignalStain® Aqueous Mounting Medium on the stained section. When the coverslip is applied, the medium will spread evenly across the coverslipped area and will dry completely. Sections may be viewed immediately, but it will take approximately 10-15 minutes for the coverslip to become immobilized and several hours to be completely hardened. c. If the view is obstructed with air bubbles or other artifacts, coverslips can be removed and sections can be remounted by soaking in buffer (PBS or TBST). Slides can be stored at room temperature for at least 12 months without the substrate fading.