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SignalStain® Boost IHC Detection Reagent (AP, Rabbit) is a ready-to-use solution intended for use in immunohistochemical assays to detect rabbit primary antibodies. SignalStain® Boost IHC Detection Reagent (AP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all alkaline phosphatase-based substrates.
Product Usage Information
1. Equilibrate SignalStain® Boost IHC Detection Reagent (AP, Rabbit) to room temperature prior to applying to the slides.2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.3. Wash slides and develop with any alkaline phosphatase-compatible substrate.
Specificity/Sensitivity
Species Reactivity:
Rabbit
SignalStain® Boost IHC Detection Reagent (AP, Rabbit) should be stored at 4°C and is stable for 12 months.
参考图片
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma (left) and gastric adenocarcinoma (right) using Vimentin (D21H3) XP® Rabbit mAb #5741 (red) and E-Cadherin (4A2) Mouse mAb #14472 (brown).SignalStain® Boost IHC Detection Reagent (AP, Rabbit) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713 were used to detect Vimentin (D21H3) XP® Rabbit mAb #5741. SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926 and SignalStain® DAB Substrate Kit #8059 were used to detect E-Cadherin (4A2) Mouse mAb #14472.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD8α (D8A8Y) Rabbit mAb #85336. Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (AP, Rabbit) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Vimentin (D21H3) XP® Rabbit mAb #5741. Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (AP, Rabbit) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713.