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SignalStain® Boost IHC Detection Reagent (AP, Mouse) is a ready-to-use solution intended for use in immunohistochemical assays to detect mouse primary antibodies. SignalStain® Boost IHC Detection Reagent (AP, Mouse) is a highly sensitive, one-step, polymer-based detection reagent that is specific for mouse IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all alkaline phosphatase-based substrates.
Product Usage Information
1. Equilibrate SignalStain® Boost IHC Detection Reagent (AP, Mouse) to room temperature prior to applying to the slides.2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.3. Wash slides and develop with any alkaline phosphatase-compatible substrate.
Specificity/Sensitivity
Species Reactivity:
Mouse
SignalStain® Boost IHC Detection Reagent (AP, Mouse) should be stored at 4°C and is stable for 12 months.
参考图片
Immunohistochemical analysis of paraffin-embedded human tonsil using CD8α (C8/144B) Mouse mAb (IHC Specific) #70306 (red) and PD-L1 (E1L3N®) XP® Rabbit mAb #13684 (brown). SignalStain® Boost IHC Detection Reagent (AP, Mouse) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713 were used to detect CD8α (C8/144B) Mouse mAb (IHC Specific). SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 and SignalStain® DAB Substrate Kit #8059 were used to detect PD-L1 (E1L3N®) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD8α (C8/144B) Mouse mAb (IHC Specific) #70306 (red) and PD-L1 (E1L3N®) XP® Rabbit mAb #13684 (brown). SignalStain® Boost IHC Detection Reagent (AP, Mouse) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713 were used to detect CD8α (C8/144B) Mouse mAb (IHC Specific). SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 and SignalStain® DAB Substrate Kit #8059 were used to detect PD-L1 (E1L3N®) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD8α (C8/144B) Mouse mAb (IHC Specific) #70306. Note: Detection was performed with SignalStain® Boost IHC Detection Reagent (AP, Mouse) and SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713.