SignalStain ® Boost IHC Detection Reagent (HRP, Goat)

SignalStain® Boost IHC Detection Reagent (HRP, Goat) is a ready-to-use solution intended for use in immunohistochemical assays to detect goat primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Goat) is a highly sensitive, one-step, polymer-based detection reagent that is specific for goat IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.

Product Usage Information

1. Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Goat) to room temperature prior to applying to the slides.2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.3. Wash slides and develop with any peroxidase-based substrate.Note: Normal goat serum cannot be used during the blocking step of the protocol. Use the Animal-Free Blocking Solution (5X) #15019 as an alternative.

Specificity/Sensitivity

Species Reactivity:

Goat