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SignalStain® Boost IHC Detection Reagent (HRP, Rat) is a ready-to-use solution intended for use in immunohistochemical assays to detect rat primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Rat) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rat IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.
Product Usage Information
1. Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Rat) to room temperature prior to applying to the slides.2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.3. Wash slides and develop with any peroxidase-based substrate.
Specificity/Sensitivity
SignalStain® Boost IHC Detection Reagent (HRP, Rat) should be stored at 4°C and is stable for 12 months.
参考图片
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using HSP70 (6B3) Rat mAb #4873 with SignalStain® Boost IHC Detection Reagent (HRP, Rat).
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using IDH1 (RcMab-1) Rat mAb #66969 with SignalStain® Boost IHC Detection Reagent (HRP, Rat).
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using TRAF1 (1F3) Rat mAb #4710 with SignalStain® Boost IHC Detection Reagent (HRP, Rat).