SignalStain ® Vivid Purple Peroxidase Substrate Kit

The SignalStain® Vivid Purple Peroxidase Substrate Kit contains all of the necessary reagents to prepare a working solution of Vivid Purple for staining tissue sections. The Vivid Purple working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, HRP, Mouse #8125, HRP, Rat #72838, and HRP, Goat #63707), yielding a purple reaction product that can provide color contrast in pigmented tissues such as melanoma.

Product Usage Information

1. To prepare the Vivid Purple working solution, to 5 ml SignalStain® Vivid Purple Diluent add 3 drops SignalStain® Vivid Purple Reagent 1, 3 drops SignalStain® Vivid Purple Reagent 2, 3 drops SignalStain® Vivid Purple Reagent 3, and 3 drops SignalStain® Vivid Purple Reagent 4. Mix well before use.2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature for 2-15 minutes. Optimal development time should be determined by the investigator.3. Wash slides for 5 minutes in water.4. Counterstain, if desired.5. Dehydrate, clear and permanently mount in a non-aqueous mounting medium. Do not use recycled alcohols with this product.Prolonged incubation in alcohol may decrease sensitivity.Unused working solution is stable up to 14 days if stored at 2-8ºC. Working solution may change color during this time with no impact on quality or intensity of the staining. If a precipitate forms, filter through a 0.45 μm filter.Appropriate personal protective equipment should be worn when handling Vivid Purple. Dispose of in accordance with local regulations.