Phospho-SLP-76 (Ser376) (D7S1K) Rabbit mAb

SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adaptor protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline-rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adaptor protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline-rich domain of SLP-76 and the SH3 domain of Itk (3). Furthermore, the proline-rich domain of SLP-76 binds to the SH3 domains of Grb2-like adaptor Gads (3,4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk, and PLCγ1 (1). The expression of SLP-76 is tightly regulated; the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5).Following TCR ligation, SLP-76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase 1 (HPK1) (6,7). This phosphorylation induces interaction with 14-3-3ε, which leads to the disassembly of TCR signaling complexes and downregulation of TCR signaling (6-8). 1.Clements, J.L. (2003) Immunol Rev 191, 211-9. 2.Bubeck Wardenburg, J. et al. (1998) Immunity 9, 607-16. 3.Bunnell, S.C. et al. (2000) J Biol Chem 275, 2219-30. 4.Liu, S.K. et al. (1999) Curr Biol 9, 67-75. 5.Clements, J.L. et al. (1998) J Immunol 161, 3880-9. 6.Shui, J.W. et al. (2007) Nat Immunol 8, 84-91. 7.Di Bartolo, V. et al. (2007) J Exp Med 204, 681-91. 8.Lasserre, R. et al. (2011) J Cell Biol 195, 839-53.