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SMAD4 (D3M6U) Rabbit mAb

品牌： CST

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参数

分子量：

反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

Smad4

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp165 of human SMAD4 protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying SMAD4. Cited in 102 publications. Validated for WB, IP, ChIP, ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, SMAD4 (D3M6U) Rabbit Monoclonal Antibody (CST #38454) is ready to ship.

商品描述

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Chromatin IP	1:100
Chromatin IP-seq	1:100

分子量

内毒素水平

犬 , 马, 豚鼠

研究领域

癌症,发育生物学与干细胞研究,纤维化,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Dog,Horse,Guinea Pig,

目标/特异性

Specificity/Sensitivity

SMAD4 (D3M6U) Rabbit mAb recognizes endogenous levels of total SMAD4 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved SSXS motif in the carboxy-terminus. Phosphorylated R-SMADS dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8). 1.Heldin, C.H. et al. (1997) Nature 390, 465-71. 2.Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94. 3.Derynck, R. et al. (1998) Cell 95, 737-40. 4.Massagué, J. (1998) Annu Rev Biochem 67, 753-91. 5.Whitman, M. (1998) Genes Dev 12, 2445-62. 6.Wrana, J.L. (2000) Sci STKE 2000, re1. 7.Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7. 8.Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

研究领域

癌症,发育生物学与干细胞研究,纤维化,神经科学,

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

4089

UniProt ID

Q13485

参考图片

Western blot analysis of extracts from various cell lines using Smad4 (D3M6U) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). HT-29 and COLO 205 are Smad4-null mutant cell lines, confirming specificity of the antibody.

Immunoprecipitation of Smad4 protein from HCT 116 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is Smad4 (D3M6U) Rabbit mAb. Western blot analysis was performed using Smad4 (D3M6U) Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ HaCaT cells treated with TGF-β1 #8915 (7 ng/mL, 1 hr) and either 5 µl of Smad4 (D3M6U) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human ID1 Promoter Primers #5139, human JunB promoter primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).

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货号：

38454T

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20ul

100ul

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