The T32-668 monoclonal antibody specifically binds to Sox9, a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox9 plays a key role in skeletal development and sex determination. Mutation of Sox9 has been associated with the human skeletal dysmorphology syndrome campomelic dysplasia, which is often characterized by both skeletal abnormalities and sex reversal. Expression of Sox9 has been observed in a variety of tissues and organs, including cartilage, testis, central neural system, lung, pancreas, intestinal stem cells, and heart.
商品描述
T32-668
The T32-668 monoclonal antibody specifically binds to Sox9, a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox9 plays a key role in skeletal development and sex determination. Mutation of Sox9 has been associated with the human skeletal dysmorphology syndrome campomelic dysplasia, which is often characterized by both skeletal abnormalities and sex reversal. Expression of Sox9 has been observed in a variety of tissues and organs, including cartilage, testis, central neural system, lung, pancreas, intestinal stem cells, and heart.
同种型
Mouse IgG1, κ
克隆号
克隆 T32-668 (RUO)
浓度
0.2 mg/ml
产品详情
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
文献
文献
研发参考(5)
1. Furuyama K, Kawaguchi Y, Akiyama H, et al. Continuous cell supply from a Sox9-expressing progenitor zone in adult liver, exocrine pancreas and intestine. Nat Genet. 2011; 43(1):34-41. (Biology).
2. Guo W, Keckesova Z, Donaher JL, et al. Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012; 148(5):1015-1028. (Biology).
3. Kent J, Wheatley SC, Andrews JE, Sinclair AH, Koopman P. A male-specific role for SOX9 in vertebrate sex determination. Development. 1996; 122(9):2813-2822. (Biology).
4. Stolt CC, Lommes P, Sock E, Chaboissier MC, Schedl A, Wegner M. The Sox9 transcription factor determines glial fate choice in the developing spinal cord. Genes Dev. 2003; 17(13):1677-1689. (Biology).
5. Wright E, Hargrave MR, Christiansen J, et al. The Sry-related gene Sox9 is expressed during chondrogenesis in mouse embryos. Nat Genet. 1995; 9(1):15-20. (Biology).
参考图片
Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma and embryonic stem cell lines. Hep G2 cells (ATCC HB-8065, Left Panel) and H9 cells (WiCell, Madison, WI; Right Panel) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No.557732; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Sox9 (Cat. No. 565493; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 or H9 cells, respectively. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma and embryonic stem cell lines. Hep G2 cells (ATCC HB-8065, Left Panel) and H9 cells (WiCell, Madison, WI; Right Panel) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No.557732; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Sox9 (Cat. No. 565493; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 or H9 cells, respectively. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
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