SPARC Like Protein 1 (SPARCL1), BioAssay™ ELISA Kit (Human)

Human

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Kit Components:96-well plate precoated with human SPARCL1 antibody, 1 Lyophilized recombinant human SPARCL1 standard, 10ng/tube×2 Biotinylated human SPARCL1 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml

Sandwich ELISA

For quantitative detection of human SPARCL1 in cell culture supernates and serum. This human SPARCL1 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been precoated onto 96-well plates. Standards(NSO, I17-F664) and test sam

Natural and recombinant human SPARCL1

46.9-3000pg/ml

Colorimetric

Cell culture supernates and serum.

<10pg/ml

8404

38220000