SPT16 (D7I2K) Rabbit mAb

Suppressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8,9). 1.Winkler, D.D. and Luger, K. (2011) J Biol Chem 286, 18369-74. 2.Orphanides, G. et al. (1999) Nature 400, 284-8. 3.Orphanides, G. et al. (1998) Cell 92, 105-16. 4.Birch, J.L. et al. (2009) EMBO J 28, 854-65. 5.Orphanides, G. and Reinberg, D. (2000) Nature 407, 471-5. 6.Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13. 7.Belotserkovskaya, R. et al. (2003) Science 301, 1090-3. 8.Schlesinger, M.B. and Formosa, T. (2000) Genetics 155, 1593-606. 9.Heo, K. et al. (2008) Mol Cell 30, 86-97.