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Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb

品牌： CST

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分子量：

反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

Src Family

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr419 of human Src protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying Yes (Tyr426) phosphate/Lyn (Tyr397) phosphate/Lck (Tyr394) phosphate/Hck (Tyr411) phosphate/Src (Tyr419) phosphate/Fyn (Tyr420) phosphate. Cited in 486 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Src Family (Tyr416) (D49G4) Rabbit Monoclonal Antibody (CST #6943) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

分子量

研究领域

癌症,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb detects endogenous levels of Src only when phosphorylated at Tyr416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It may cross react with overexpressed phosphorylated RTKs.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). 1.Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609. 2.Hunter, T. (1987) Cell 49, 1-4.

研究领域

癌症,代谢,神经科学

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

7525,4067,3932,3055,6714,2534

UniProt ID

P07947,P07948,P06239,P08631,P12931,P06241

参考图片

Western blot analysis of extracts NIH/3T3 cells, serum-starved or treated with human Platelet-Derived Growth Factor BB hPDGF-BB #8912 (100 ng/ml, 15 min), using Phospho-Src Family (Tyr416) (D49G8) Rabbit mAb (upper) or Src (36D10) Rabbit mAb #2109 (lower).

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货号：

6943T

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