首页抗体一抗一抗

Stat1 Antibody

1/4

Stat1 Antibody

品牌： CST

下载产品说明书

下载COA

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

分子量：

反应种属：

展开

产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

Stat1

来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Stat1. Antibodies are purified by protein A and peptide affinity chromatography.

宿主

Rabbit

商品描述

Product Usage Information

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Chromatin IP	1:50

分子量

84, 91

内毒素水平

牛 , 犬

研究领域

免疫学和肿瘤学,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Bovine,Dog,

目标/特异性

Specificity/Sensitivity

Stat1 Antibody detects endogenous levels of total Stat1 protein. The antibody detects both Stat1alpha (91kDa) and Stat1beta (84 kDa) isoforms.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. 1.Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120. 2.Durbin, J.E. et al. (1996) Cell 84, 443-50. 3.Meraz, M.A. et al. (1996) Cell 84, 431-42. 4.Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7. 5.Frank, D.A. (1999) Mol Med 5, 432-56. 6.Wen, Z. et al. (1995) Cell 82, 241-50.

研究领域

免疫学和肿瘤学,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

6772

UniProt ID

P42224

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either Stat1 Antibody or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western blot analysis of extracts from A549 cells (lane 1) or STAT1 knock-out cells (lane 2) using Stat1 Antibody #9172 (upper), and β-actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal in the STAT1 knock-out A549 cells confirms specificity of the antibody for STAT1.

Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with non-targeted (control) siRNA or transfection with Stat1 siRNA. Stat1 was detected using Stat1 Antibody #9172, and p42 was detected using p42 MAPK Antibody #9108. The Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity.

Western blot analysis of extracts from SK-MEL-28 cells, untreated or IFN-alpha-treated (100 ng/ml), using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) or Stat1 Antibody (lower).

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

9172S

一键复制

询价

优宁维现货

20ul

100ul

300ul

选择数量

当前规格1件起购

配送至

预计3-4个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价