Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb (Alexa Fluor ® 647 Conjugate)

Stat2 (113 kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5). 1.Fu, X.Y. et al. (1992) Proc Natl Acad Sci U S A 89, 7840-3. 2.Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7. 3.Park, C. et al. (2000) Immunity 13, 795-804. 4.Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80. 5.Horvath, C.M. et al. (1996) Mol Cell Biol 16, 6957-64.