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产品介绍
产品信息
抗原名称
STING
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu328 of human STING protein.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying STING. Cited in 471 publications. Validated for WB, IP, IHC, IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, STING (D2P2F) Rabbit Monoclonal Antibody (CST #13647) is ready to ship.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
分子量
33, 35
研究领域
免疫学和肿瘤学,神经科学,
应用
反应种属
Human,Mouse,
目标/特异性
Specificity/Sensitivity
STING (D2P2F) Rabbit mAb recognizes endogenous levels of total STING protein.
Species Reactivity:
Human, Mouse, Hamster
敏感性
Endogenous
背景
背景
Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
1.Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
2.Zhong, B. et al. (2008) Immunity 29, 538-50.
3.Sun, L. et al. (2013) Science 339, 786-91.
4.Wu, J. et al. (2013) Science 339, 826-30.
5.Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
6.Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
7.Ishikawa, H. et al. (2009) Nature 461, 788-92.
研究领域
免疫学和肿瘤学,神经科学,
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #67733.
数据库链接
Entrez-Gene ID
340061
UniProt ID
Q86WV6
参考图片
Western blot analysis of extracts from various cell lines using STING (D2P2F) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-), transfected with a construct expressing human STING protein (hSTING; +), or transfected with a construct expressing mouse STING protein (mSTING; +), using STING (D2P2F) Rabbit mAb.
Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb.
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