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STING (D2P2F) Rabbit mAb

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STING (D2P2F) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu328 of human STING protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
IHC Leica Bond	1:100 - 1:400
Immunohistochemistry (Paraffin)	1:50 - 1:200

同种型

Rabbit IgG

分子量

33, 35

应用

目标/特异性

Specificity/Sensitivity

STING (D2P2F) Rabbit mAb recognizes endogenous levels of total STING protein.

Species Reactivity:

Human, Mouse, Hamster

敏感性

Endogenous

背景

Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7). 1.Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8. 2.Zhong, B. et al. (2008) Immunity 29, 538-50. 3.Sun, L. et al. (2013) Science 339, 786-91. 4.Wu, J. et al. (2013) Science 339, 826-30. 5.Zhang, Z. et al. (2011) Nat Immunol 12, 959-65. 6.Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004. 7.Ishikawa, H. et al. (2009) Nature 461, 788-92.

研究领域

免疫学和肿瘤学,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #67733.

数据库链接

Entrez-Gene ID

340061

UniProt ID

Q86WV6

参考图片

Immunohistochemical analysis of paraffin-embedded HT-29 (left, positive) and A549 (right, negative) cell pellets using STING (D2P2F) Rabbit mAb.

Western blot analysis of extracts from various cell lines using STING (D2P2F) Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-), transfected with a construct expressing human STING protein (hSTING; +), or transfected with a construct expressing mouse STING protein (mSTING; +), using STING (D2P2F) Rabbit mAb.

Western blot analysis of PMA-treated THP-1 extracts from WT (left) or STING1 KO (right) using STING (D2P2F) Rabbit mAb. Membranes stained with Ponceau S for total protein normalization (lower). These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies, as a companion to validation data generated by CST scientists.

Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using STING (D2P2F) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human neuroendocrine lung carcinoma using STING (D2P2F) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin’s lymphoma using STING (D2P2F) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using STING (D2P2F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using STING (D2P2F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using STING (D2P2F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using STING (D2P2F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse lung using STING (D2P2F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Syrian hamster uterus using STING (D2P2F) Rabbit mAb.

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货号：

13647T

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