BD Pharmingen™ AKP Streptavidin

Streptavidin

Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Alkaline Phosphatase (AKP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, AKP Streptavidin has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.

Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Alkaline Phosphatase (AKP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, AKP Streptavidin has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.

(RUO)

Immunohistochemistry (Routinely Tested)

Streptavidin

Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.