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BD Pharmingen™ AKP Streptavidin
品牌： BD Pharmingen
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咨询实验应用：
Immunohistochemistry (Routinely Tested)
产品介绍
产品介绍
产品信息
抗原名称
Streptavidin
简单描述
Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium
Streptomyces avidinii
. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Alkaline Phosphatase (AKP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, AKP Streptavidin has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.
商品描述
Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium
Streptomyces avidinii
. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Alkaline Phosphatase (AKP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, AKP Streptavidin has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.
克隆号
(RUO)
产品详情
AKP
Alkaline phosphatase (AKP) is an enzyme that is capable of dephosphorylating various substrates which generates a measurable colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with AKP and used in applications such as i) ELISA that employs a plate reader to measure absorbance of the colored product or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
应用
实验应用
Immunohistochemistry (Routinely Tested)
目标/特异性
Streptavidin
制备和贮存
存储溶液
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
参考图片
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