BD Pharmingen- AKP Streptavidin_BD Pharmingen

抗原名称

Streptavidin

简单描述

Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain molecules expressed by cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Alkaline Phosphatase (AKP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, AKP Streptavidin has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry. This reagent conjugate can be used as a detecting antibody in ELISA; a dilution of 1:1000 is recommended (1:500 if glycerol has been added). For immunohistochemical staining, we recommend the use of BD Pharmingen™ AKP Streptavidin (Cat. No. 551008) that is specially formulated for immunohistochemistry. AKP Streptavidin is a useful second-step reagent for labeling a biotinylated primary antibody for detection by ELISA (with an appropriate substrate system).

商品描述

Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain molecules expressed by cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Alkaline Phosphatase (AKP) and coupled with the use of a colorimetric, luminescent, or fluorescent substrate development system, AKP Streptavidin has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry. This reagent conjugate can be used as a detecting antibody in ELISA; a dilution of 1:1000 is recommended (1:500 if glycerol has been added). For immunohistochemical staining, we recommend the use of BD Pharmingen™ AKP Streptavidin (Cat. No. 551008) that is specially formulated for immunohistochemistry. AKP Streptavidin is a useful second-step reagent for labeling a biotinylated primary antibody for detection by ELISA (with an appropriate substrate system).

克隆号

(RUO)

产品详情

AKP

Alkaline phosphatase (AKP) is an enzyme that is capable of dephosphorylating various substrates which generates a measurable colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with AKP and used in applications such as i) ELISA that employs a plate reader to measure absorbance of the colored product or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.