BD™ ELISPOT HRP Streptavidin for ELISPOT

Streptavidin

Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules to stain target molecules expressed by cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Horseradish Peroxidase (HRP) and coupled with the use of  a colorimetric, luminescent, or fluorescent substrate development system, Streptavidin HRP has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.

Streptavidin is a non-glycosylated protein that is purified chromatographically from the bacterium Streptomyces avidinii . Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated primary or secondary antibodies and other biotinylated specific-binding molecules to stain target molecules expressed by cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. When conjugated with an enzyme such as Horseradish Peroxidase (HRP) and coupled with the use of  a colorimetric, luminescent, or fluorescent substrate development system, Streptavidin HRP has found widespread use along with biotinylated primary or secondary antibodies in a number of applications including Western blot, ELISA, ELISPOT, Immunocytochemistry and Immunohistochemistry.

(RUO)

ELISPOT (Routinely Tested)

Streptavidin

Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.