Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium
Streptomyces avidinii
. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, BD™ ELISPOT, immunocytochemistry and immunohistochemistry.
Streptavidin is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
商品描述
Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium
Streptomyces avidinii
. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, BD™ ELISPOT, immunocytochemistry and immunohistochemistry.
Streptavidin is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
克隆号
(RUO)
浓度
0.2 mg/ml
产品详情
PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Flow cytometric comparison of CD45R/B220 expression on mouse splenocytes.
Left Panel: BALB/c mouse spleen cells were stained with Biotin Rat anti-Mouse CD45R/B220 (Cat. No. 553086) antibody followed by BD Horizon™ PE-CF594 Streptavidin (Cat. No. 562284/562318; solid line histogram). The dashed line histogram overlay represents staining on splenocytes with PE-CF594 Streptavidin alone.
Right Panel: BALB/c mouse spleen cells were stained with Biotin Rat anti-Mouse CD45R/B220 antibody followed by either BD Horizon™ PE-CF594 Streptavidin (solid line histogram) or BD Pharmingen™ PE-Texas Red™ Streptavidin (Cat. No. 551487, dashed line histogram). Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric comparison of CD45R/B220 expression on mouse splenocytes using Biotin-conjugated Anti-Mouse CD45R/B220 antibody and either BD Horizon™ PE-CF594 Streptavidin or PE-Texas Red Streptavidin. Left Panel: BALB/c mouse spleen cells were stained with Biotin Rat anti-Mouse CD45R/B220 (Cat. No. 553086) antibody followed by BD Horizon™ PE-CF594 Streptavidin (Cat. No. 562284/562318; black line histogram). The dashed line histogram overlay represents staining on splenocytes with PE-CF594 Streptavidin alone. Right Panel: BALB/c mouse spleen cells were stained with Biotin Rat anti-Mouse CD45R/B220 antibody followed by either BD Horizon™ PE-CF594 Streptavidin (black line histogram) or BD Pharmingen™ PE-Texas Red™ Streptavidin (Cat. No. 551487, dashed line histogram overlay). Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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